g (2pts) A 10x transfer buffer solution is 250mM Tris and 1.92M glycine. Buffers are always used at 1x concentration in the lab (unless specified otherwise in the protocol), so we will have to dilute the 10x buffer to 1x before use. What is the concentration of Tris and glycine in the 1x buffer

Answers

Answer 1

Answer:

The explanation according to the given question is summarized below.

Explanation:

Given:

Tris,

= 250 mM

Glycine,

= 1.92 M

According to the solution,

For the dilution pf 10X to 1X buffer, we get

= [tex]1 \ ml \ of \ 10X \ buffer +9 \ ml \ of \ distilled \ water[/tex]

= [tex]10[/tex]

i.e.,

⇒ [tex]10X \ to \ 1X=1:10 \ dilution[/tex]

Now,

⇒ [tex]10X (250 \ mM\ Tris \ HCl, 1.92M\ Glycine, and\ 1 \ percent (\frac{w}{v} ) SDS) ---->1X(25 \ mM \ Tris \ HCl,0.193 M\ Glycine, and \ 0.1 \ percent(\frac{w}{v} )SDS)[/tex]


Related Questions

What happens when 10g of nitrogen combines with 10 grams of hydrogen

Answers

It would create 12.1 g of ammonia (NH3).

The amount of hydrogen that is reacted in this reaction is:

(10 g N2)[tex](\frac{3.0gH2}{14.0gN2}) =[/tex] 2 g H2

According to the Law of Conservation of Mass, the mass of ammonia produced is  the combination of hydrogen and nitrogen reacted or:

2.1g + 10.0g = 12.1 g of NH3

Pure magnesium metal is often found as ribbons and can easily burn in the presence of oxygen. When 3.31 g of magnesium ribbon burns with 8.45 g of oxygen, a bright, white light and a white, powdery product are formed. Enter the balanced chemical equation for this reaction. Be sure to include all physical states.

Answers

Answer:

2Mg(s) + O2(g) ----->2MgO(s)

Explanation:

In writing a balanced chemical reaction equation, the rule of thumb is that the number of atoms of each element on the reactant side must be the same as the number of atoms of the same elements on the products side.

Hence for the reaction of magnesium and oxygen shown below;

2Mg(s) + O2(g) ----->2MgO(s)

We notice that there are two atoms each of magnesium and oxygen on both sides of the reaction equation hence the equation is balanced.

PLS HELP
When 50.0 g of copper was reacted with 300.0 g of silver nitrate solution, 149 g of silver was obtained. What is the percent yield of silver obtained?

Answers

Answer:

Percent yield of silver obtained = 78.18% (Approx.)

Explanation:

Given:

Amount of copper = 50 gram

Amount of silver nitrate = 300 gram

Amount of silver = 149 gram

Find:

Percent yield of silver obtained

Computation:

Percentage of Silver in silver nitrate =  108[100/(108+14+48)]

Percentage of Silver in silver nitrate = 108/[100/170]

Percentage of Silver in silver nitrate = 63.53% (Approx.)

Amount of Silver produced = [63.53%][300]

Amount of Silver produced = 190.59 gram

Percent yield of silver obtained = [Amount of silver / Amount of silver produced]100

Percent yield of silver obtained = [149/190.59]100

Percent yield of silver obtained = 78.18% (Approx.)

which element is found in period 6 group 14

Answers

Answer:

Lead

that can be found in the periodic table of elements

Explanation:

The half-life of phosphorous-32 is 14.28 days. How many days are required for 4.68 g to remain if you start with 74.88
grams

Answers

57.167days will be required for 4.68g to remain


How many moles of H20 are produced from 3 moles of oxygen?
2 H2+O2 -> 2 H2O

Answers

Answer:

6 moles

Explanation:

because if conducting a moles ratio for oxygen and water it is 1:2 so the moles would calculate up to 6 moles after cross multiplication.

What is the volume of a bubble if the original was .25 m^3 at 4 atm and the pressure was reduced to 1 atm?

Answers

Answer:

1.00 m^3

Explanation:

Boyles law says that PV = constant, or P1V1=P2V2, while temperature remains constant.

0.25 m^3 *4 atm = V* 1 atm

V = 0.25 m^3 / (1/4)

= 1 m^3

i. What is this process of forming energy through combustion called when it occurs in the body?

Answers

Answer:

Respiration

Explanation:

Energy, steam and carbondioxide formed.

If 10.0 moles of NO are reacted with 6.0 moles of O2, How many moles of NO2 are produced?​

Answers

Answer:

We have to find out which of the reactants that is the Limiting Reagent.

The Limiting Reagent is the reactant that finishes before reaction is complete or simply put... Its the reactant that's NOT IN EXCESS.

We use the reactant that's not in excess to find the Number of moles of product because once that reactant is used up.... The reaction also ends or stops(hence the name;Limiting reactant).

Now

We have 10moles of NO

We also have 6moles of O2

The balanced equation of Reaction is

2NO + O2 ===> 2NO2

Now

looking at this equation

2moles of NO is required to react with 1mole of O2

So since we have NO=10moles and O2 = 6moles

It means that

20moles of NO is required to react with 6 moles of O2.

We were only given 10moles of NO and this is not even enough to react with the whole 6moles of Oxygen because from what we just discovered... We need 20moles of NO to react with all 6moles of O2.

So

This means that NO is the LIMITING REACTANT. It is in short supply and would be used up before the whole reaction takes place.

So we use the limiting reactant to find the Number of Moles of Product formed.

Now

From the eqn of reaction

2moles of NO forms 2Moles of NO2.

Their Mole ratio is 2:2 or 1:1...

This simply means that an equal amount of NO2 will be Produced

MEANING.... 10 MOLES OF NO2 IS PRODUCED.

HAVE A GREAT DAY!!!

7. In science, the process/thing that you examine/focus on is called the ??
and anything outside of this focus is called the ??

Answers

Answer:

Subject of the experiment and out of scope

Explanation:

In any research study, the process of thing studied is basically the subject of the experiment on which research is conducted to determine the outcomes. If any thing lies out of the experimental process reach, then it is said to be out of scope of the experiment.

How many grams of P2O3 are needed to react with 248 g of NaOH ?

Answers

Answer:

170.43g P2O3

Explanation:

First write and balance the equation:

P2O3 + 4NaOH → 2Na2HPO3 + H2O

NaOH=39.997 g/mol  P2O3= 109.945 g/mol

248g NaOH x 1 mol NaOH/39.997g NaOH x 1 mol P2O3/4 mol NaOH x

109.945g P2O3/ 1 mol P2O3 = 170.43g P2O3

pcl3 give me brief explanation donot obey octet rule? I will give him brainlist?​

Answers

Answer:

Explanation:

PCl3, the octet for both phosphorus and Chlorine atoms is complete. Therefore, it follows the octet rule.

Name the substance which occurs in all the physical states in nature?​

Answers

Answer: water

For many centuries, it was considered that there were only three states of matter: solid, liquid and gas (the three that are present and stable in our world). And water is the substance that best represents them, because it is the only one that exists naturally in the three states.

Explanation: Matter can exist in one of three main states: solid, liquid, or gas.

Solid matter is composed of tightly packed particles. ...

Liquid matter is made of more loosely packed particles. ...

Gaseous matter is composed of particles packed so loosely that it has neither a defined shape nor a defined volume.

Answer:

Water is the only common substance that is naturally found as a solid, liquid or gas. Solids, liquids and gases are known as states of matter.

Feel free to mark it as brainliest :D

Ignore this part unless you want to understand out of your syllabus:

However, if you take into account the fifth state of matter, Bose-Einstein Condensate, which has been recently discovered, there is no substance as such.

Consider the reaction 4FeS2 + 11O2 → 2Fe2O3 + 8SO2. If 8 moles of FeS2 react with 15 moles of O2, what is the limiting reactant? (3 points)


SO2

O2

Fe2O3

FeS2

Answers

Answer:

O2

Explanation:

for find the limiting reactant you must calculate the moles of the reactants from the amount that you have and from the MM:

MM FeS2 = 120n  = 26.2g / 120g/mol = 0,218 mol

MM O2  = 32n = 5,44g/32g/mol = 0,17 mol

The limiting reactant is  

O2

Jared has a genetic disorder.
which is the most likely reason Jared has the disorder?

Answers

Answer:

parents / genetics

Explanation:

a genetic disorder most likely comes from a family relative weather it being mom,dad, grandpa, uncle,ect.

Question 2
4 pts
Inside a car that was at STP, a 0.95 L bottle warms up to 144 C. If the volume of the bottle
remains constant, what is the pressure, in atm, inside the hot water bottle?

Answers

Answer:

1.53 atm

Explanation:

From the question given above, the following data were obtained:

Volume = constant

Initial pressure (P₁) = stp = 1 atm

Initial temperature (T₁) = 273 K

Final temperature (T₂) = 144 °C = 144 °C + 273 = 417 K

Final pressure (P₂) =?

Since the volume is constant, the final pressure can be obtained as follow:

P₁ / T₁ = P₂ / T₂

1 / 273 = P₂ / 417

Cross multiply

273 × P₂ = 417

Divide both side by 273

P₂ = 417 / 273

P₂ = 1.53 atm

Therefore, the final pressure (i.e the pressure inside the hot water bottle) is 1.53 atm.

A more electronegative atom A) will have more attraction to the electrons in a chemical bond. B) is more likely to lose an electron. C) is less likely to form a chemical bond. D) is more likely to form an ionic bond with another highly electronegative atom.

Answers

Answer: A more electronegative atom will have more attraction to the electrons in a chemical bond.

Explanation:

An atom that is able to attract electrons or shared pair of electrons more towards itself is called an electronegative atom.

For example, fluorine is the most electronegative atom.

Due to its high electronegativity it is able to attract an electropositive atom like H towards itself. As a result, both fluorine and hydrogen will acquire stability by sharing of electrons.

Thus, we can conclude that a more electronegative atom will have more attraction to the electrons in a chemical bond.

please help ASAPPPPPPPPPPPPPPP

Answers

d AHHAHAHA DNKWPAKHEBW

hello HELP ME pLS ANYONE with this question I will give u 18 points pls I need real help here:((​

Answers

Answer:

Boiling - The process of a liquid changing to gas.

Boiling point - The temperature at which a liquid boils.

Condensing - The process of a gas changing to a liquid.

Distillate - The liquid collect from the condenser.

Residue - The liquid left behind in the distillation flask.

Vapour - Another name for gas.

Hope it helps :)

When aqueous solutions of potassium nitrite and nitric acid are mixed, an aqueous solution of potassium nitrate and nitrous acid results. Write the net ionic equation for the reaction.

Answers

1.) C2H3O2-(aq) + H+(aq) ===> HC2H3O2(aq). K+ and Cl- are spectator ions. 2.) Ni(s) + Sn2+(aq) ===> Ni2+(aq) + Sr(s). NO3- is a spectator ion. 3.) 3Ag+ + PO4(3-)(aq) ===> Ag3(PO4)(s). Na+ and NO3-

Nitric acid and potassium nitrite reacted together potassium nitrate and nitrous acid forms. The net ionic equation for the reaction is given below:

[tex]\rm K^{+} (aq)+ NO_{3} ^{-} (aq)\rightarrow KNO_{3}(s)[/tex]

What is ionic equation?

A balanced reaction represents all the reactants and products in perfect stoichiometry. Thus, each element have to be equal in number on both side.

A balanced ionic equation represents all the ions participating in a reaction and with their charges balanced on both side and state of the reaction is given in brackets. Net ionic equation represents the formation of a solid precipitate.

The complete ionic equation of reaction of one mole of potassium nitrite with one mole of nitric acid is given below:

[tex]\rm K^{+} + NO_{2}^{-}(aq) + H^{+} (aq) +NO_{3}^{-}(aq) \rightarrow H^{+} (aq) + NO_{2}^{-}(aq) + KNO_{3} (s)[/tex]

Cancelling the aqueous ions from both sides we get the net ionic equation representing the formation of solid potassium nitrate as shown here:

[tex]\rm K^{+} (aq)+ NO_{3} ^{-} (aq)\rightarrow KNO_{3}(s)[/tex]

To find more on ionic equations, refer here:

https://brainly.com/question/15467502

#SPJ5

How many moles are in 152 grams of H20?

Answers

Answer:

8.437282129392383

Explanation:

We assume you are converting between grams Water and mole.

You can view more details on each measurement unit:

molecular weight of Water or mol

The molecular formula for Water is H2O.

The SI base unit for amount of substance is the mole.

1 grams Water is equal to 0.055508435061792 mole.

Note that rounding errors may occur, so always check the results.

Answer:

8.44moles

Explanation:

8.44moles

Why hydrogen iodide is not produced by the same method is for HCL.​

Answers

Answer:

acidic in the order. HBr and HI can't be prepared like H

They ic in the order . HBr & HI can,t be prepared like HCl by using Concentrated

Answer:

acidic in the order. HBr and HI can't be prepared like H

They ic in the order . HBr & HI can,t be prepared like HCl by using Concentrated

Which piece of glassware shown below is used to hold and dispense a solution of known concentration during a titration?

Answers

Answer:

Answer C

Explanation:

Got it off of q**zlet ;)

Answer

Its C confirmed

Explanation:

Just did the test

a. how many grams of H2SO4 are in 3.5 liters of 2.0 M H2SO4

b. how many liters of 2.0 M H2SO4 would be made from 3 liters of water. (Hint water has a density of 1g per mL.)

Answers

Answer:

a. 686g of H2SO4 are present.

b. 3.321L would be made

Explanation:

a. Molarity, M, is an unit of concentration defined as the ratio between moles of solute (In this case H2SO4) and liters of solution. The moles of H2SO4 in 3.5L of 2.0M H2SO4 are:

3.5L * (2.0mol H2SO4 / L) = 7.0 moles H2SO4

The mass is obtained using molar mass of H2SO4 -98g/mol-:

7.0 moles H2SO4 * (98g / mol) = 686g of H2SO4 are present

b. The density of H2SO4 is 1.83g/mL. The volume of 2.0M H2SO4 per liter of solution is:

2.0mol H2SO4 / L * (98g / mol) * (1mL / 1.83g) = 107mL of H2SO4 are present per liter of solution. In 3L there are:

3L  (107mL / L) = 321mL = 0.321L of H2SO4 must be added to 3L of water to produce 2.0M H2SO4. The total volume is:

3L + 0.321L = 3.321L would be made

suggest me some good korean or Thai or English movie

for example I am suggesting - 8th graders don't cry , so young 2 never gone , Cinderella 2015, Titanic , slumdog millionaire... ​

Answers

Answer:

try to see THE PIXELS, AVENGERS, JOKER, CHARLIE'S ANGEL, JUSTICE LEAGUE,IT ,THE NUN ETC

Answer:

watch movie COCO and croods a new age

You start with 0.1 L of a 5.00 M solution, and you plan to dilute it to make a
solution with a concentration of 0.625 M. How much water did you add to
the container?
a) 800ML
b) 100ml
c)700ml
d)750ml

Answers

You add 100 ml of water to the container

The largest pieces being dragged along the streambed are undergoing the process called ________. pushing saltation traction suspension solution

Answers

Answer:

saltation

Explanation:

Saltation is the transportation of particles by fluids such as by winds and water.  It takes place when loose matter is removed from the sea bed and gets carried by the fluid back to the surface. Examples include pebbles, sand, and soil. Saltation layers can form during avalanches.

how many grams of cacl2 can be produced from 50.0 grams of hcl

Answers

Answer:

76.0 g

Explanation:

Step 1: Write the balanced equation

2 HCl + Ca(OH)₂ ⇒ CaCl₂ + 2 H₂O

Step 2: Calculate the moles corresponding to 50.0 g of HCl

The molar mass of HCl is 36.46 g/mol.

50.0 g × 1 mol/36.46 g = 1.37 mol

Step 3: Calculate the number of moles of CaCl₂ produced from 1.37 moles of HCl

The molar ratio of HCl to CaCl₂ is 2:1. The moles of CaCl₂ produced are 1/2 × 1.37 mol = 0.685 mol.

Step 4: Calculate the mass corresponding to 0.685 moles of CaCl₂

The molar mass of CaCl₂ is 110.98 g/mol.

0.685 mol × 110.98 g/mol = 76.0 g

When light is refracted
A.
it changes its direction as it passes into a new medium.

B.
it changes it direction and stays in the same medium.

C.
it keeps moving in the same direction as it passes into a new medium.

D.
it is absorbed as it strikes the surface of a new medium.

Answers

[tex]\huge{ \mathfrak{  \underline{ Answer }}}[/tex]

Refraction is a phenomenon in which, light bends itself while moving from from one Medium to another. This occurs due to change in speed of light in different mediums.

Therefore correct option is :

A. it changes its direction as it passes into a new medium.

I hope it helped you !!

[tex] \boxed{ \boxed{\huge{ \mathrm{  \underline{ Answer} \:  \:  }}}}[/tex]

The Correct option will be : -

A. it changes its direction as it passes into a new medium.

because, light travels with different speeds in different mediums, and this occurs due to difference in optical density of different mediums.

And bending of light depends upon the change in medium,

for example :

when travelling from optically denser medium to optically rarer medium it bends away from normal.

when travelling from optically rarer medium to optically denser medium it bends towards the normal.

_____________________________

[tex]\mathrm{ ☠ \: TeeNForeveR \: ☠}[/tex]

Which change to an object would double its kinetic energy?
A. Increasing its velocity to twice its original value
B. Increasing its mass to four times its original value
C. Increasing its mass to twice its original value
Ο Ο
D. Increasing its velocity to four times its original value

Answers

Answer:

Increasing its mass to twice its original value

Explanation:

Pls mark me brainliest

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