Researchers isolated a yeast phosphofructokinase mutant in which a serine at the fructose-2,6-bisphosphate (F26BP) binding site was replaced with an aspartate residue. The amino acid substitution completely abolished the binding of F26BP to PFK. There was a dramatic decline in glucose consumption and ethanol production in the mutant compared to control yeast.

Required:
a. Propose a hypothesis that explains why the mutant PFK cannot bind F26BP.
b. What does the decline of glucose consumption and ethanol production in the yeast reveal about the role of F26BP in glycolysis?

Answers

Answer 1

Answer:

Explanation:

a.

Phosphofructokinase-1, also known as the glycolysis stimulator, is among the most important glycolysis regulatory enzymes. It's an apoptotic enzyme with effectors (activators/inhibitors) that control its activity. Fructose 6-phosphate and ATP are converted to fructose 1,6-bisphosphate and ADP by PFK-1.

Suppose the serine in PFK's active site is substituted with aspartic acid, the enzyme's active site would be devoid of phosphorylation sites. The enzyme PFK contributes to the transferase family since it has kinase activity. It serves as an acceptor by transferring phosphorus groups to an alcohol group (serine).

Insulin stimulates protein phosphatase, which eliminates the phosphate group from the serine residue, stabilizing the kinase role and promoting F26BP formation. The phosphorylation reaction will not take place provided the serine is substituted with aspartic acid. Therefore, the PFK's kinase activity will be lost. Similarly, F26BP will not form PFK, and it will not be aborted in the future.

b.

Their decline reveals that:

F26BP increases the overall net flow of glucose by glycolysis modulates PFK production and induces Fructose 1,6 bis phosphatase, which also catalyzes the glycolysis reverse process.

Fructose-2,6-bisphosphate transcriptionally activates the rate-limiting process of glycolysis catalyzed by PFK 1.

Increased levels of Fru-2,6-P2 located in the liver raise PFK1's propensity and resistance for fructose 6 phosphate while lowering its affinity for its' inhibitors ATP and citrate.

Fructose 2,6-bisphosphate supersedes the inhibition of ATP and promotes greater sensitivity of the eukaryotes to be regulated by hormones (glucagon and insulin).


Related Questions

A template strand of DNA in a gene reads:
TCT CCA AGC 5
Using the codon chart below, what is the sequence of amino acids that is produced after translation?

(Choice A
Ser - Gly - Gly

(Choice B)
B
Ser - Gly - Leu

(Choice C)
C
Arg - Ser - Gly

(Choice D)
D
Arg - Gly- Ser

Answers

Answer:

(Choice D)

Arg - Gly - Ser

Which of the following processes can help dalry farmers generate a substantial amount of methane from the manure
from their herds?

A. fermentation
B. krebs cycle
C. aerobic respiration
D. glycolysis

Answers

A. fermentation thank

A student crosses a pure-breeding line of red-flowered poinsettias with a pure-breeding line of white-flowered poinsettias. The student observes that all the plants in the F1 generation have pink flowers. The student then crosses the F1 plants with one another and records 64 red-flowered plants, 107 pink-flowered plants and 53 white-flowered plants. What is the inheritance pattern for flower color in these poinsettias

Answers

Answer:

The correct answer is - incomplete dominance.

Explanation:

The incomplete dominance is the pattern of inheritance where the recessive allele is not completely masked by the dominant allele in the heterozygous condition and makes a mixed or blend expression of both dominant and recessive traits.

In the given question, the pattern for the flower color is incomplete dominance as in the cross between the red and white flower poinsettias produce pink instead of red in F1 generation where the condition is heterozygous. The phenotypic ratio of in F2 generation in this type of inheritance is 1:2:1 which is approximately similar in this case 64:107:53.

Answer:

The calculated chi-square value is 1.53, and the null hypothesis cannot be rejected.

Explanation:

write a letter to your friend telling her how you are coping in your new senior school​

Answers

Answer:

Dear Seb,

                     Thank you for your letter, it means a lot to me seeing as though i'm still transitioning into my new school and finding friends. I miss you and all of our friends back home. it's funny, even though i have been gone for months I still refer to California as home rather than New York. It's senior year, the closing chapter of my childhood and I feel as though I have started all over again. I try to remain optimistic, but I feel like my entire life was disturbed since I moved away. I have been trying to get myself involved as much as possible for a new senior class student. An idle mind is not complacent and I find myself in a rut whenever I am not doing anything. Keeping myself busy has distracted me from my constant stress, and anxiety when I think about how far I am from everything I know and love. My current motivator is the chance that I could get accepted into a UC school and go back home. I miss you.

                                                     Love always,

                                                                             Jasmine

Explanation:

Why should we not pollute the earth

Answers

Answer: Reducing pollutants in the air is important for human health and the environment. Poor air quality has harmful effects on human health, particularly the respiratory and cardiovascular systems. Pollutants can also damage plants and buildings, and smoke or haze can reduce visibility.

One reason why we should stop pollution is that pollution is destroying the environment we live in. Power stations produce nearly 10 billion tons of carbon dioxide per year. Carbon dioxide is a big contribution for pollution. This shows that the water, pollution, air pollution and ground pollution are common.

An estuary collects sediments from the ocean and rivers that feed into it. The sediments swirl around and then settle to form a mudflat. Eel grass is then established on the mudflat. The ecosystem changes over time and ultimately develops into a salt marsh that contains mangrove trees.
Which of the following is likely NOT involved in this example of ecological succession?

A)
The rotting remains of plants add to the fertility of the soil.


B)
The soil becomes so fertile that eel grass is replaced by other plant species.


C)
The roots from plants help stabilize the sediment, keeping it in place.


D)
The concentration of salt becomes so high that all plant life is destroyed.

Can y'all included detail answers on why the other answers are incorrect

Answers

I’m pretty sure it’s A

The pressure you exert on the floor decreases when you stand on your toes because the area on which you exert force decreases.


Please select the best answer from the choices provided

T
F

Answers

False..

Your exert weight should be the same

A Rectal Enema is used to treat
Constipation. This would represent
what type of drug delivery?
Select one:
O a. Enteral and systemic
O b. Parenteral and local
O c. Enteral and local
O d. Parenteral and systemic​

Answers

Answer:

The correct answer is - C. Enteral and local.

Explanation:

Which of the following is not produced by cellular respiration?
Options:
water
carbon dioxide
ATP energy
oxygen

Answers

Answer:

The one above is not the right answer, oxygen is the correct answer

calculate the quantity of electrical energy used by an electrical bulb rated 60 Walt's used for 2hours​

Answers

Answer:

0.12KWH

Explanation:

Time: 2hrs

Power: 60watt

60/1000

Formula: Energy=potential/time

0.06×2= 0.12KWH

What are some potentially linked genes you think humans might have?

Answers

Answer:

The potentially linked genes are the x or the y chromosomes.

Explanation:

And some of the more familiar sex linked traits are hemophilia, red-green blindness, and congenital night blindness.

Define Haploid
Define Diploid
What is the number of chromosomes for diploid human cells?
What is the number of chromosomes for haploid pea plant cells?​

Answers

Answer:

Define Haploid

1 set of chromosomes.

Define Diploid

2 sets of chromosomes.

What is the number of chromosomes for diploid human cells?

46

What is the number of chromosomes for haploid pea plant cells?

7

-TheUnknownScientist

Explanation:

number of chromosomes for diploid human cells is 46.

number of chromosomes for haploid pea plant cells is 7

please help no links or i will report

Answers

Answer and Explanation:

The difference between a food chain and a food web:

Food Chain

- Only one possible direct line of energy going through organisms

- Consists of usually 4-6 organisms

Food Web

- Multiple links  that branch off in a web-like form, with some organisms being interconnected with each other.

The answer is the third option: A food chain represents one possible pathway that energy and matter can take in an ecosystem. A food web contains multiple, interconnected food chains and describes how energy and matter moves through an entire ecosystem.

#teamtrees #PAW (Plant And Water)

What bacteria structures can make bacteria RESISTANT TO ANTIBIOTICS. CHOOSE ALL THAT APPLY.
Flagella
Cell wall
Outer membrane
Endospore
Plasmid
Capsule

Answers

Cell Wall

Explanation:

About 63% of the bacteria tested were Gram-negative, which means that they have a cell wall that is more resistant to certain classes of antibiotics than the cell walls of Gram-positive bacteria.

In terms of plant life, this biome is extremely high in diversity, but there are very few animals that live here.

tru or false?

Answers

Answer:

Tundra

Explanation:

i belive its tundra babe

True. The statement "In terms of plant life, this biome is extremely high in diversity, but there are very few animals that live here" accurately describes a tropical rainforest biome.

What is tropical rainforest biome?

Tropical rainforest biome is known for its high level of plant biodiversity and relatively low number of animal species compared to other biomes. Tropical rainforests are home to a wide variety of plant species, including trees, shrubs, and epiphytes, but the dense vegetation and high rainfall make it difficult for many animal species to establish themselves.

However, the few species that are able to survive in the rainforest typically have specialized adaptations that allow them to thrive in this unique environment.

Learn more about tropical rainforest biome, here:

https://brainly.com/question/15340927

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Natata Townsend
1. Which of the following nutrient is responsible for repairing of cells?
O (a) calcium
O (e) fats
O (b) protein
0 (d) carbohydrates​

Answers

Answer:

B

Explanation:

the nutrient that is required for repairing cells is protein

what is the difference between sex chromosomes found in humna females and human males?

Answers

Answer:

Females have XX sex chromosomes while males have XY sex chromosomes.

( THIS IS SCIENCE WORK ) HELP ASAP PLS I NEED HELP NO LINKS PLS
Explain in your own words the order in which the creature's traits may have evolved, starting
from the first likely trait. (Hint: Which trait is most common across the creatures?)

Answers

Answer:

Use the following key to help you complete the field study.

HINT: You can view the creatures by clicking on the link HERE.  You may also use the activity on Page 5 of the lesson in order to view them.

Step

Physical Feature

Classification

1

Smooth skin

Go to 2

Fur

Go to 3

2

One eye

Go to 4

Two eyes

Go to 5

3

One eye

Go to 7

Two eyes

Go to 8

4

Arms and legs

Silkus monowrestle

Legs only

Silkus monosquirmus

5

Arms and legs

Go to 6

Legs only

Silkus duosquirmus

6

Tail

Silkus tallyhas

No tail

Silkus stretchilus

7

Tail

Fuzzus tallywag

No tail

Fuzzus feelzalot

8

Two teeth

Go to 9

More than two teeth

Fuzzus chompilus

9

Pointy

Fuzzus pointilus

Square

Fuzzus squarilus

this is the lesson

List seven features that you can use to identify the depositional environment in which sedimentary rocks
formed

Answers

Answer:

Sedimentary rocks are types of rock that are formed by the accumulation or deposition of mineral or organic particles at the Earth's surface, followed by cementation

The seven features that we can use to identify the depositional environment in which sedimentary rocks are formed are stratification, cross-beds, graded bedding, ripple marks, mud cracks, fossils, and concretions.

What are sedimentary rocks?

Sedimentary rocks are created by the materials being deposited at the Earth's surface and then being cemented at their positions by water bodies.

There are four ways that these rocks are created:

Deposition of the other rocksAccumulation of the sedimentsDeposition of the results of biogenic activityPrecipitation from solution.

Examples of sedimentary rocks are:

LimestoneShale ChalkSandstoneClay

They are used to making cement, glass, building stones and act as energy sources.

Learn more about sedimentary rocks, here:

https://brainly.com/question/24112914

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give two advantage of ploughing the field ​

Answers

Advantages of ploughing the soil are: - Tilling of soil helps in loosening and turning off the soil, the nutrient-rich soil is distributed evenly and also it improves aeration. ... - Improvement in the microbiome of the soil. - Roots can easily penetrate the soil and provide a stronger foothold for the plant.

In polygenic inheritance, each of the genes controlling a trait will have exactly the same influence over the trait. Group of answer choices Yes since all genes having to do with a trait always have the exact same influence on a trait. No since different genes will exert varying degrees of influence on the trait None of the other responses are satisfactory answers. No since all traits are only controlled by one gene with two alleles.

Answers

Answer:

No, since different genes will exert varying degrees of influence on the trait

Explanation:

The term quantitative heritability refers to the transmission of a phenotypic trait in which expression depends on the additive effect of a series of genes.

Polygenic heritability occurs when a trait results from the interaction of more than one gene. And these genes can also have more than two alleles. The action of many genes and alleles can cause many different combinations that are the reason for genotypic graduation.

Quantitative traits are those that can be measure, such as longitude, weight, eggs laid per female, among others. These characters do not group individuals by any precise and clear categories. Instead, they group individuals in many different categories that depend on how the genes were intercrossed and distributed during meiosis. The result depends on the magnitude in which each allele contributes to the final phenotype and genotype. When they interact, they create a gradation in phenotypes, according to the level of contribution.  

Let us see, for instance, the eye color trait, which results from the interaction of many genes. Two of them significantly contribute to the color green, blue, or brown, while the rest of the involved genes play a role in defining the spectrum of phenotypes of each eye color. So, there are different tones of blue eyes, green eyes, and brown eyes, and the differences are determined by the contribution of each allele to the phenotype.

hich term represents animals with backbones?

invertebrates
heterotrophs
autotrophs
vertebrates

Answers

Answer:

Vertebrates represents animals with backbones.

Answer:

D. Vertebrates

Explanation:

Is it true that if one part of the brain is damaged all body functions will be affected

Answers

Answer:Sometimes only a single area is affected, but in most cases of TBI multiple areas have been injured. When all areas of the brain are affected, the injury can be very severe.

so yes

Explanation:

Answer:

In some cases

Explanation:

In some cases hiting specific parts of the body can restrict or damage all body functions, but those cases are rare. It is more likely that if a part of the brain is damage only a few specific function are affected.

Brainliest?

define SELECTIVE BREEDING in your own words

Answers

Answer:

Selective breeding is when humans use animal breeding and plant breeding to create phenotypic characteristics by picking which animal or plant genders will reproduce together.

Explanation:

hope this helped!

what organisms create energy for other organisms to consume

Answers

Answer:

Autotrophs

Explanation:

Autotrophs are organisms that use energy directly from the sun or from chemical bonds. Commonly called producers, they use the energy and simple inorganic compounds to produce organic molecules. Autotrophs are vital to all ecosystems because all organisms need organic molecules and only autotrophs can produce them from inorganic compounds. There are two basic types of autotrophs: photoautotrophs and chemoautotrophs.

Photoautotrophs

Photoautotrophs are autotrophs that use energy from sunlight to make organic compounds by photosynthesis. Photoautotrophs include plants, algae, and many bacteria, as shown in the figure below. They are the primary producers in the vast majority of ecosystems on Earth.

Chemoautotrophs

Chemoautotrophs use energy from chemical bonds to make organic compounds by chemosynthesis. Chemoautotrophs include certain bacteria and archaeans. They are the primary producers in ecosystems that form around hydrothermal vents and in hot springs.

Answer:

Autotrophs

Explanation:

Autotrophs are the only type of organism that produce energy for other organism.

However, there are different types of autotrophs that do 2 different things to produce their food.

First off, autotrophs are made up of fungi, algae, bacteria, and more commonly, plants.

They produce their own food two different ways,

The first way, and most commonly known, is photosythesis.

This is where a mix of carbon dioxide, water, and sunlight undergo 2 complex proccess to make glucose.

This is the energy that consumers, known as heterotrophs take from the autotrophs.

The second and less commonly known way of producing food is chemosythesis.

In chemosytrhesis, carbon dioxide or methane which is transformed into nutrients through the oxidization of things such as hydrogen gas and hydrogen sulfure, whihc are mainly found deep in the ocean in hydrothermal vents.

So, to answer your question:

Autrophs produce their own energy

Or more specific:

Plants, fungi, bacteria, and algae produce their own energy.

Hope this helps! :)

Losing baby teeth is an example of the
dimension of development.
A. moral
B. psychosocial
C. cognitive
D. physical

Answers

Losing baby teeth is an example of the physical dimension of development. So, it's D.

What do you mean by Physical development?

Physical development may be defined as the transition of the character or traits of humans between birth and adolescence.

Any transition of value may be considered under moral development. Psychosocial development involves the ability to understand the perspective of others on the basis of own knowledge and desires.

Cognitive development involves the adjustment of your characters with infants that including rhymes, stories, singing, etc.

Therefore, the correct option for this question is D.

To learn more about Physical development, refer to the link:

https://brainly.com/question/12822960

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what brought great wealth to the Inca empire
Having gold and sliver
exporting coffee
trading with Spanish
finding oil and natural gas

Answers

Gold and silver is correct as the Spanish were the ones who conquered the Inca empire if I am remembering that correctly but definitely A

what should be the daily routine labor requirements in the farm plot? Cite at least 5 daily labor requirements?​

Answers

Answer:

Explanation:

Labor requirements are the tasks carry out in farms for effective production and these include;

1. Watering...the farmer or worker is expected to water the plants so that they will absorb water for effective growth..

2. Fertilization application for improve yield

3. Weeding to reduce competition for nutrients, space and water with crops.

4. Prunning

5. Harvesting of matured crops.

6 . Transporting of harvested crops.

5 daily requirements in a farm plot are:

Watering of plantsWeedingFertilizinguse of pesticidesTransportation

What is a farm plot?

This is an area of land that is used for agricultural purposes. A farm plot usually has one or more crops planted on the soil.

The farmers has to water the crops daily to ensure that the crops grow well. He also has to weed to reduce competition for the crops.

Using fertilizers helps to improve the yields and pesticides drives unwanted insects from the crops.

Read more on a farm plot here: https://brainly.com/question/10336353

Which of the following statements correctly describes the relationship between erosion and deposition?

A.
Erosion breaks rocks into pieces, and deposition moves the pieces.

B.
Deposition and erosion both involve the movement of small pieces of rock from one place to another.

C.
Erosion is the movement of small pieces of rock from one place to another, and deposition is when the rock settles in a new location.

D.
Deposition and erosion break rocks into smaller pieces, but the rocks remain in place.

Answers

Answer:

I think It's A

Explanation:

I hope I helped :)

3.Some plants have structures or features that protect them from harm.

If a plant has bitter-tasting leaves, what would this most likely protect the plant against?Lector inmersivo
(1 Punto)

caterpillars that would eat the plant

cold weather that would cause the plant to freeze

people who would pick the plant for its pretty flowers

hot weather that would cause the leaves to dry up

Answers

Answer:

Caterpillars that would eat the plant

Explanation: